Seronegative Spondyloarthritis and its association with HLA B27
Madhumita Mukhopadhyay*and Shivangi Kailash
ABSTRACT
Introduction: Seronegative Spondyloarthritis comprises of various arthritis like rheumatoid arthritis, ankylosing spondylitis, inflammatory arthritis, reactive arthritis and etc. Seronegative spondyloarthritis is a frequently observed condition. Most of these cases are HLA B27 positive.
Aims and Objectives: The aim of our study was to assess the association of the HLA B27 antigen in the population of Seronegative Spondyloarthritis and also distribution of Seronegative Spondyloarthritis in different age group.
Materials and Methods: The study was carried out in the Pathology department in a Tertiary Care Hospital. The patients attending in the department of Pathology from August 2016 to August 2023 with Seronegative Spondyloarthritis for evaluation of HLA B27 were included in the study. EDTA blood was collected. Then DNA extraction was done manually by DNA extraction kit. The HLA B27 were assessed by Real time PCR ( RTPCR ) by the help of kit.
Results: Out of total 615 patients with seronegative arthritis 446 (72%) patients were positive for HLA B27. The male patients outnumbered the females. 461 (75% ) were male and the most frequent age group was 16 to 50 year. Low back pain and cervical pain were most prevalent complaints. The most common extraarticular manifestation was uveitis (red eye), seen in 89 (20%) patients. The most frequent MRI finding was Sacroilitis. 423 (95%) out of 446 HLA B27 positive patients presented with Sacroilitis.
Conclusion: Seronegative Spondyloarthritis is often associated with HLA B27. It is found mainly in male. Most common age group was 16- 50 year.
