Determination of Atenolol in Pharmaceutical Preparations and Environmental Wastewater Samples: Application to Content Uniformity Testing
Nief Rahman Ahmad* and Ghfran Naif Rahman
ABSTRACT
A simple, accurate, precise, rapid, economical and sensitive Uv spectrophotometric method has been developed for the determination of Atenolol in pharmaceutical preparations and environmental wastewater samples, which shows maximum absorbance at 225 nm in methanol. Beer's law was obeyed in the range of 2.5 -30 μg/ ml, with molar absorptivity of 9.9877×103 L.mol-1.cm-1, relative standard deviation of the method was less than 1.6%, and accuracy (average recovery %) was 100 ± 1.2. No interference was observed from common excipients and additives often accompany with Atenolol in pharmaceutical preparations. The method was successfully applied to the determination of Atenolol in some pharmaceutical formulations (tablets) and industrial wastewater samples. The proposed method was validated by sensitivity and precision which proves suitability for the routine analysis of Atenolol in true samples.
